Journal: International Journal of Women's Health

Article Title: Prediction of Cervical Cancer Progression Leveraging HPV16 Integration-Related Genes

doi: 10.2147/IJWH.S543345

Figure Lengend Snippet: NRF2 signaling inhibition sensitizes CC cells to cisplatin treatment. ( A ) IC50 values of HeLa, SiHa and C33A cells treated with cisplatin for 48 h. The data are representative of 3 biological replicates. ( B ) IC50 values of HeLa, SiHa and C33A cells treated with cisplatin for 48 h. Cells were pretreated with ML385 (1 µM) for 2 hours prior to cisplatin exposure, with ML385 maintained throughout the entire treatment period. The data are representative of 3 biological replicates. ( C ) RT-qPCR showing the mRNA expression of NQO1 in CC cell lines. Cells were pretreated with ML385 (1 µM) for 2 hours prior to cisplatin exposure. The data are representative of 3 biological replicates. ( D ) Representative histograms and gMFI of DCFH-DA in SiHa and HeLa cells. The data are representative of 3 biological replicates. *p< 0.05, **p< 0.01, ***p< 0.001.

Article Snippet: Human CC cell line HeLa, SiHa and C33A were obtained from the American Type Culture Collection (ATCC).

Techniques: Inhibition, Quantitative RT-PCR, Expressing

Journal: Frontiers in Medicine

Article Title: Multi-omics profiling reveals the role of 4-ethylbenzoic acid in promoting proliferation and invasion of cervical cancer

doi: 10.3389/fmed.2025.1591531

Figure Lengend Snippet: 4-EA plays a promoting role in the growth and migration of cervical cancer cells. (A) The concentration of 4-EA in vaginal lavage fluids in people with different health conditions by targeted metabolomics. (B) Cell viability of HeLa and SiHa cells after 4-EA treatment was detected by CCK-8 assay. (C,D) Cell proliferation was examined by EdU assay. (E,F) The clone ability of HeLa and SiHa cells was determined by colony formation assay with 4-EA treatment. Data are presented as the mean ± SD. * p < 0.05; ** p < 0.01; *** p < 0.001. NILM, negative for intraepithelial lesion or malignancy; LSIL, low-grade squamous intraepithelial lesion; HSIL, high-grade squamous intraepithelial lesion; CC, cervical cancer.

Article Snippet: CC cell lines HeLa and SiHa (Procell Life Science & Technology Co., Ltd., Wuhan, China) were purchased and maintained in DMEM (Gibco; Thermo Fisher Scientific, New York, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco) and 1% penicillin–streptomycin (Gibco), and incubated at 37 °C with 5% CO2.

Techniques: Migration, Concentration Assay, CCK-8 Assay, EdU Assay, Colony Assay